Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CRTC1 / MECT1 / TORC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113288-100
Catalog Number: LS-G113288
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TORC1(CRTC1) (NM_015321), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRTC1 | KIAA0616 | MECT1 | TORC-1 | TORC1 | Transducer of CREB protein 1 | WAMTP1
- Origin Species
- Human
- NCBI GenBank
- NM_001098482
- SwissProt
- Q6UUV9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATSNNPRKF SEKIALHNQK QAEETAAFEE VMKDLSLTRA ARLQLQKSQY LQLGPSRGQY61YGGSLPNVNQ IGSGTMDLPF QTPFQSSGLD TSRTTRHHGL VDRVYRERGR LGSPHRRPLS121VDKHGRQADS CPYGTMYLSP PADTSWRRTN SDSALHQSTM TPTQPESFSS GSQDVHQKRV181LLLTVPGMEE TTSEADKNLS KQAWDTKKTG SRPKSCEVPG INIFPSADQE NTTALIPATH241NTGGSLPDLT NIHFPSPLPT PLDPEEPTFP ALSSSSSTGN LAANLTHLGI GGAGQGMSTP301GSSPQHRPAG VSPLSLSTEA RRQQASPALS PLSPITQAVA MDALSLEQQL PYAFFTQAGS361QQPPPQPQPP PPPPPASQQP PPPPPPQAPV RLPPGGPLLP SASLTRGPQP PPLAVTVPSS421LPQSPPENPG QPSMGIDIAS APALQQYRTS AGSPANQSPT SPVSNQGFSP GSSPQHTSTL481GSVFGDAYYE QQMAARQANA LSHQLEQFNM MENAISSSSL YSPGSTLNYS QAAMMGLTGS541HGSLPDSQQL GYASHSGIPN IILTVTGESP PSLSKELTSS LAGVGDVSFD SDSQFPLDEL601KIDPLTLDGL HMLNDPDMVL ADPATEDTFR MDRLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
634 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.