Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPEB1 / CPEB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120092-100
Catalog Number: LS-G120092
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPEB1 (NM_001079534), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CPE-BP1 | CPEB1 | CPE-binding protein 1 | H-CEBP | HCPEB-1 | CEBP | CPEB
- Origin Species
- Human
- NCBI GenBank
- NM_030594
- SwissProt
- Q9BZB8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLFPTSAQES SRGLPDANDL CLGLQSLSLT GWDRPWSTQD SDSSAQSSTH SVLSMLHNPL61GNVLGKPPLS FLPLDPLGSD LVDKFPAPSV RGSRLDTRPI LDSRSSSPSD SDTSGFSSGS121DHLSDLISSL RISPPLPFLS LSGGGPRDPL KMGVGSRMDQ EQAALAAVTP SPTSASKRWP181GASVWPSWDL LEAPKDPFSI EREARLHRQA AAVNEATCTW SGQLPPRNYK NPIYSCKVFL241GGVPWDITEA GLVNTFRVFG SLSVEWPGKD GKHPRCPPKG YVYLVFELEK SVRSLLQACS301HDPLSPDGLS EYYFKMSSRR MRCKEVQVIP WVLADSNFVR SPSQRLDPSR TVFVGALHGM361LNAEALAAIL NDLFGGVVYA GIDTDKHKYP IGSGRVTFNN QRSYLKAVSA AFVEIKTTKF421TKKVQIDPYL EDSLCHICSS QPGPFFCRDQ VCFKYFCRSC WHWRHSMEGL RHHSPLMRNQ481KNRDSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.