Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPEB1 / CPEB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109483-100
Catalog Number: LS-G109483
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPEB1 (NM_030594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CPE-BP1 | CPEB1 | CPE-binding protein 1 | H-CEBP | HCPEB-1 | CEBP | CPEB
- Origin Species
- Human
- NCBI GenBank
- NM_030594
- SwissProt
- Q9BZB8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAFPLEEEAG RIKDCWDNQE APALSTCSNA NIFRRINAIL DNSLDFSRVC TTPINRGIHD61HLPDFQDSEE TVTSRMLFPT SAQESSRGLP DANDLCLGLQ SLSLTGWDRP WSTQDSDSSA121QSSTHSVLSM LHNPLGNVLG KPPLSFLPLD PLGSDLVDKF PAPSVRGSRL DTRPILDSRS181SSPSDSDTSG FSSGSDHLSD LISSLRISPP LPFLSLSGGG PRDPLKMGVG SRMDQEQAAL241AAVTPSPTSA SKRWPGASVW PSWDLLEAPK DPFSIEREAR LHRQAAAVNE ATCTWSGQLP301PRNYKNPIYS CKVFLGGVPW DITEAGLVNT FRVFGSLSVE WPGKDGKHPR CPPKGYVYLV361FELEKSVRSL LQACSHDPLS PDGLSEYYFK MSSRRMRCKE VQVIPWVLAD SNFVRSPSQR421LDPSRTVFVG ALHGMLNAEA LAAILNDLFG GVVYAGIDTD KHKYPIGSGR VTFNNQRSYL481KAVSAAFVEI KTTKFTKKVQ IDPYLEDSLC HICSSQPGPF FCRDQVCFKY FCRSCWHWRH541SMEGLRHHSP LMRNQKNRDS STRTRPLEQKLISEEDLAANDILDYKDDDDKV
561 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.