LSBio
CNTN4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNTN4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNTN4 antibodies →
$725.00each
Catalog Number: LS-G104982-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNTN4 (NM_175607), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AXCAM | Contactin 4 | Contactin-4 | BIG-2 | CNTN4
Origin Species
Human
SwissProt
Q8IWV2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLPWELLVL QSFILCLADD STLHGPIFIQ EPSPVMFPLD SEEKKVKLNC EVKGNPKPHI
61RWKLNGTDVD TGMDFRYSVV EGSLLINNPN KTQDAGTYQC TATNSFGTIV SREAKLQFAY
121LDNFKTRTRS TVSVRRGQGM VLLCGPPPHS GELSYAWIFN EYPSYQDNRR FVSQETGNLY
181IAKVEKSDVG NYTCVVTNTV TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPTAKG
241ATVKLECFAL GNPVPTIIWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GLYECVAENS
301RGKNVARGQL TFYAQPNWIQ KINDIHVAME ENVFWECKAN GRPKPTYKWL KNGEPLLTRD
361RIQIEQGTLN ITIVNLSDAG MYQCLAENKH GVIFSNAELS VIAVGPDFSR TLLKRVTLVK
421VGGEVVIECK PKASPKPVYT WKKGRDILKE NERITISEDG NLRIINVTKS DAGSYTCIAT
481NHFGTASSTG NLVVKDPTRV MVPPSSMDVT VGESIVLPCQ VTHDHSLDIV FTWSFNGHLI
541DFDRDGDHFE RVGGQDSAGD LMIRNIQLKH AGKYVCMVQT SVDRLSAAAD LIVRGPPGPP
601EAVTIDEITD TTAQLSWRPG PDNHSPITMY VIQARTPFSV GWQAVSTVPE LIDGKTFTAT
661VVGLNPWVEY EFRTVAANVI GIGEPSRPSE KRRTEEALPE VTPANVSGGG GSKSELVITW
721ETVPEELQNG RGFGYVVAFR PYGKMIWMLT VLASADASRY VFRNESVHPF SPFEVKVGVF
781NNKGEGPFSP TTVVYSAEEE PTKPPASIFA RSLSATDIEV FWASPLEKNR GRIQGYEVKY
841WRHEDKEENA RKIRTVGNQT STKITNLKGS VLYHLAVKAY NSAGTGPSSA TVNVTTRKPP
901PSQPPGNIIW NSSDSKIILN WDQVKALDNE SEVKGYKVLY RWNRQSSTSV IETNKTSVEL
961SLPFDEDYII EIKPFSDGGD GSSSEQIRIP KISNAYARGS GASTSNACTL SAISTIMISL
1021TARSSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1026 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.