Western blot analysis of extracts of various cell lines, using CNTN4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

CNTN4 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human
Format:	Liquid

$281.00each

Catalog Number: LS-C497047-20

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Overview

Description: CNTN4 antibody LS-C497047 is an unconjugated rabbit polyclonal antibody to human CNTN4. Validated for WB.
Application: WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: AXCAM | Contactin 4 | Contactin-4 | BIG-2 | CNTN4
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q8IWV2
NCBI GenBank: NP_783200.1
SwissProt: Q8IWV2

Target

Target: Human CNTN4
Antigen Species: Human
Gene Name: CNTN4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 448-697 of human CNTN4 (NP_001193885.1). VGVFNNKGEGPFSPTTVVYSAEEEPTKPPASIFARSLSATDIEVFWASPLEKNRGRIQGYEVKYWRHEDKEENARKIRTVGNQTSTKITNLKGSVLYHLAVKAYNSAGTGPSSATVNVTTRKPPPSQPPGNIIWNSSDSKIILNWDQVKALDNESEVKGYKVLYRWNRQSSTSVIETNKTSVELSLPFDEDYIIEIKPFSDGGDGSSSEQIRIPKISNAYARGSGASTSNACTLSAISTIMISLTARSSL
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)