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CMIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CMIP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129122-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CMIP (NM_198390), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C-Maf-inducing protein | C-Mip | CMIP | KIAA1694 | TCMIP | Tc-Mip | C-Maf inducing protein
Origin Species
Human
NCBI GenBank
NM_030629
SwissProt
Q8IY22

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVTSSSGGG GDPRQIEETK PLLGGDVSAP EGTKMGAVPC RRALLLCNGM RYKLLQEGDI
61QVCVIRHPRT FLSKILTSKF LRRWEPHHLT LADNSLASAT PTGYMENSVS YSAIEDVQLL
121SWENAPKYCL QLTIPGGTVL LQAANSYLRD QWFHSLQWKK KIYKYKKVLS NPSRWEVVLK
181EIRTLVDMAL TSPLQDDSIN QAPLEIVSKL LSENTNLTTQ EHENIIVAIA PLLENNHPPP
241DLCEFFCKHC RERPRSMVVI EVFTPVVQRI LKHNMDFGKC PRLRLFTQEY ILALNELNAG
301MEVVKKFIQS MHGPTGHCPH PRVLPNLVAV CLAAIYSCYE EFINSRDNSP SLKEIRNGCQ
361QPCDRKPTLP LRLLHPSPDL VSQEATLSEA RLKSVVVASS EIHVEVERTS TAKPALTASA
421GNDSEPNLID CLMVSPACST MSIELGPQAD RTLGCYVEIL KLLSDYDDWR PSLASLLQPI
481PFPKEALAHE KFTKELKYVI QRFAEDPRQE VHSCLLSVRA GKDGWFQLYS PGGVACDDDG
541ELFASMVHIL MGSCYKTKKF LLSLAENKLG PCMLLALRGN QTMVEILCLM LEYNIIDNND
601TQLQIISTLE STDVGKRMYE QLCDRQRELK ELQRKGGPTR LTLPSKSTDA DLARLLSSGS
661FGNLENLSLA FTNVTSACAE HLIKLPSLKQ LNLWSTQFGD AGLRLLSEHL TMLQVLNLCE
721TPVTDAGLLA LSSMKSLCSL NMNSTKLSAD TYEDLKAKLP NLKEVDVRYT EAW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

773 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.