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CMIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CMIP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109427-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CMIP (NM_030629), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C-Maf-inducing protein | C-Mip | CMIP | KIAA1694 | TCMIP | Tc-Mip | C-Maf inducing protein
Origin Species
Human
NCBI GenBank
NM_030629
SwissProt
Q8IY22

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQAAEPTGY MENSVSYSAI EDVQLLSWEN APKYCLQLTI PGGTVLLQAA NSYLRDQWFH
61SLQWKKKIYK YKKVLSNPSR WEVVLKEIRT LVDMALTSPL QDDSINQAPL EIVSKLLSEN
121TNLTTQEHEN IIVAIAPLLE NNHPPPDLCE FFCKHCRERP RSMVVIEVFT PVVQRILKHN
181MDFGKCPRLR LFTQEYILAL NELNAGMEVV KKFIQSMHGP TGHCPHPRVL PNLVAVCLAA
241IYSCYEEFIN SRDNSPSLKE IRNGCQQPCD RKPTLPLRLL HPSPDLVSQE ATLSEARLKS
301VVVASSEIHV EVERTSTAKP ALTASAGNDS EPNLIDCLMV SPACSTMSIE LGPQADRTLG
361CYVEILKLLS DYDDWRPSLA SLLQPIPFPK EALAHEKFTK ELKYVIQRFA EDPRQEVHSC
421LLSVRAGKDG WFQLYSPGGV ACDDDGELFA SMVHILMGSC YKTKKFLLSL AENKLGPCML
481LALRGNQTMV EILCLMLEYN IIDNNDTQLQ IISTLESTDV GKRMYEQLCD RQRELKELQR
541KGGPTRLTLP SKSTDADLAR LLSSGSFGNL ENLSLAFTNV TSACAEHLIK LPSLKQLNLW
601STQFGDAGLR LLSEHLTMLQ VLNLCETPVT DAGLLALSSM KSLCSLNMNS TKLSADTYED
661LKAKLPNLKE VDVRYTEAW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

679 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.