Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CES2 / Esterase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103851-100
Catalog Number: LS-G103851
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CES2 (NM_198061), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Carboxylesterase 2 | CES2 | CE-2 | CES2A1 | Cocaine esterase | Esterase | ICE | HCE-2 | PCE-2
- Origin Species
- Human
- NCBI GenBank
- NM_003869
- SwissProt
- O00748
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAQSRSPTT PTFPGPSQRT PLTPCPVQTP RLGKALIHCW TDPGQPLGEQ QRVRRQRTET61SEPTMRLHRL RARLSAVACG LLLLLVRGQG QDSASPIRTT HTGQVLGSLV HVKGANAGVQ121TFLGIPFAKP PLGPLRFAPP EPPESWSGVR DGTTHPAMCL QDLTAVESEF LSQFNMTFPS181DSMSEDCLYL SIYTPAHSHE GSNLPVMVWI HGGALVFGMA SLYDGSMLAA LENVVVVIIQ241YRLGVLGFFS TGDKHATGNW GYLDQVAALR WVQQNIAHFG GNPDRVTIFG ESAGGTSVSS301LVVSPISQGL FHGAIMESGV ALLPGLIASS ADVISTVVAN LSACDQVDSE ALVGCLRGKS361KEEILAINKP FKMIPGVVDG VFLPRHPQEL LASADFQPVP SIVGVNNNEF GWLIPKVMRI421YDTQKEMDRE ASQAALQKML TLLMLPPTFG DLLREEYIGD NGDPQTLQAQ FQEMMADSMF481VIPALQVAHF QCSRAPVYFY EFQHQPSWLK NIRPPHMKAD HVKFTEEEEQ LSRKMMKYWA541NFARNGNPNG EGLPHWPLFD QEEQYLQLNL QPAVGRALKA HRLQFWKKAL PQKIQELEEP601EERHTELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
607 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.