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CDYL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CDYL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CDYL antibodies →
$504.00each
Catalog Number: LS-G105597-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDYL (NM_170751), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDY-like | CDYL | Chromodomain Y-like protein | autosomal | CDYL1 | Chromodomain protein | Y-like | DKFZP586C1622
Origin Species
Human
NCBI GenBank
NM_004824
SwissProt
Q9Y232

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASEELYEVE RIVDKRKNKK GKTEYLVRWK GYDSEDDTWE PEQHLVNCEE YIHDFNRRHT
61EKQKESTLTR TNRTSPNNAR KQISRSTNSN FSKTSPKALV IGKDHESKNS QLFAASQKFR
121KNTAPSLSSR KNMDLAKSGI KILVPKSPVK SRTAVDGFQS ESPEKLDPVE QGQEDTVAPE
181VAAEKPVGAL LGPGAERARM GSRPRIHPLV PQVPGPVTAA MATGLAVNGK GTSPFMDALT
241ANGTTNIQTS VTGVTASKRK FIDDRRDQPF DKRLRFSVRQ TESAYRYRDI VVRKQDGFTH
301ILLSTKSSEN NSLNPEVMRE VQSALSTAAA DDSKLVLLSA VGSVFCCGLD FIYFIRRLTD
361DRKRESTKMA EAIRNFVNTF IQFKKPIIVA VNGPAIGLGA SILPLCDVVW ANEKAWFQTP
421YTTFGQSPDG CSTVMFPKIM GGASANEMLL SGRKLTAQEA CGKGLVSQVF WPGTFTQEVM
481VRIKELASCN PVVLEESKAL VRCNMKMELE QANERECEVL KKIWGSAQGM DSMLKYLQRK
541IDEF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

544 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.