Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDH16 / Cadherin 16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116901-100
Catalog Number: LS-G116901
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cadherin 16(CDH16) (NM_004062), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin 16 | KSP-cadherin | Cadherin-16 | CDH16 | Kidney-specific cadherin
- Origin Species
- Human
- NCBI GenBank
- NM_004062
- SwissProt
- O75309
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 89.92 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVPAWLWLLC VSVPQALPKA QPAELSVEVP ENYGGNFPLY LTKLPLPREG AEGQIVLSGD61SGKATEGPFA MDPDSGFLLV TRALDREEQA EYQLQVTLEM QDGHVLWGPQ PVLVHVKDEN121DQVPHFSQAI YRARLSRGTR PGIPFLFLEA SDRDEPGTAN SDLRFHILSQ APAQPSPDMF181QLEPRLGALA LSPKGSTSLD HALERTYQLL VQVKDMGDQA SGHQATATVE VSIIESTWVS241LEPIHLAENL KVLYPHHMAQ VHWSGGDVHY HLESHPPGPF EVNAEGNLYV TRELDREAQA301EYLLQVRAQN SHGEDYAAPL ELHVLVMDEN DNVPICPPRD PTVSIPELSP PGTEVTRLSA361EDADAPGSPN SHVVYQLLSP EPEDGVEGRA FQVDPTSGSV TLGVLPLRAG QNILLLVLAM421DLAGAEGGFS STCEVEVAVT DINDHAPEFI TSQIGPISLP EDVEPGTLVA MLTAIDADLE481PAFRLMDFAI ERGDTEGTFG LDWEPDSGHV RLRLCKNLSY EAAPSHEVVV VVQSVAKLVG541PGPGPGATAT VTVLVERVMP PPKLDQESYE ASVPISAPAG SFLLTIQPSD PISRTLRFSL601VNDSEGWLCI EKFSGEVHTA QSLQGAQPGD TYTVLVEAQD TDEPRLSASA PLVIHFLKAP661PAPALTLAPV PSQYLCTPRQ DHGLIVSGPS KDPDLASGHG PYSFTLGPNP TVQRDWRLQT721LNGSHAYLTL ALHWVEPREH IIPVVVSHNA QMWQLLVRVI VCRCNVEGQC MRKVGRMKGM781PTKLSAVGIL VGTLVAIGIF LILIFTHWTM SRKKDPDQPA DSVPLKATVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
829 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.