Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CDCA1 / NUF2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109226-100
Catalog Number: LS-G109226
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NUF2 (NM_031423), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cancer/testis antigen 106 | CDCA1 | HNuf2 | NUF2 | Kinetochore protein Nuf2 | NUF2R | CT106 | HNuf2R | HsNuf2
- Origin Species
- Human
- NCBI GenBank
- NM_031423
- SwissProt
- Q9BZD4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METLSFPRYN VAEIVIHIRN KILTGADGKN LTKNDLYPNP KPEVLHMIYM RALQIVYGIR61LEHFYMMPVN SEVMYPHLME GFLPFSNLVT HLDSFLPICR VNDFETADIL CPKAKRTSRF121LSGIINFIHF REACRETYME FLWQYKSSAD KMQQLNAAHQ EALMKLERLD SVPVEEQEEF181KQLSDGIQEL QQSLNQDFHQ KTIVLQEGNS QKKSNISEKT KRLNELKLSV VSLKEIQESL241KTKIVDSPEK LKNYKEKMKD TVQKLKNARQ EVVEKYEIYG DSVDCLPSCQ LEVQLYQKKI301QDLSDNREKL ASILKESLNL EDQIESDESE LKKLKTEENS FKRLMIVKKE KLATAQFKIN361KKHEDVKQYK RTVIEDCNKV QEKRGAVYER VTTINQEIQK IKLGIQQLKD AAEREKLKSQ421EIFLNLKTAL EKYHDGIEKA AEDSYAKIDE KTAELKRKMF KMSTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
464 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.