Detection of Human and Mouse NUF2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
CDCA1 / NUF2 Antibody (aa414-464)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C290959-10
Catalog Number: LS-C290959
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- Description
- NUF2 antibody LS-C290959 is an unconjugated rabbit polyclonal antibody to NUF2 (CDCA1) (aa414-464) from human. It is reactive with human and mouse. Validated for IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Cancer/testis antigen 106 | CDCA1 | HNuf2 | NUF2 | Kinetochore protein Nuf2 | NUF2R | CT106 | HNuf2R | HsNuf2
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- Q9BZD4
- NCBI GenBank
- NM_031423 | NP_113611.2
- SwissProt
- Q9BZD4
Target
- Target
- Human CDCA1 / NUF2
- Antigen Species
- Human
- Epitope
- Region between residue 414 to 464 of human Nuclear Fallout 2 using the numbering given in entry NP_663735.2 (GeneID 83540).
- Gene Name
- NUF2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 83540 corresponding to amino acids between 414 and 464 of UniprotID: Q9BZD4
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate