Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CD66a / CEACAM1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129804-100
Catalog Number: LS-G129804
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEACAM1 (NM_001184813), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antigen CD66 | BGP1 | Biliary glycoprotein 1 | CD66a | BGP-1 | CEACAM1 | BGP | BGPI | CD66a antigen
- Origin Species
- Human
- NCBI GenBank
- NM_001712
- SwissProt
- P13688
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGHLSAPLHR VRVPWQGLLL TASLLTFWNP PTTAQLTTES MPFNVAEGKE VLLLVHNLPQ61QLFGYSWYKG ERVDGNRQIV GYAIGTQQAT PGPANSGRET IYPNASLLIQ NVTQNDTGFY121TLQVIKSDLV NEEATGQFHV YPELPKPSIS SNNSNPVEDK DAVAFTCEPE TQDTTYLWWI181NNQSLPVSPR LQLSNGNRTL TLLSVTRNDT GPYECEIQNP VSANRSDPVT LNVTYGPDTP241TISPSDTYYR PGANLSLSCY AASNPPAQYS WLINGTFQQS TQELFIPNIT VNNSGSYTCH301ANNSVTGCNR TTVKTIIVTD NALPQENGLS PGAIAGIVIG VVALVALIAV ALACFLHFGK361TGRASDQRDL TEHKPSVSNH TQDHSNDPPN KMNEVTYSTL NFEAQQPTQP TSASPSLTAT421EIIYSEVKKQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
430 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.