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CCT6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCT6B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCT6B antibodies →
$504.00each
Catalog Number: LS-G130991-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCT6B (NM_001193529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCTZ-2 | CCT-zeta-2 | CCT-zeta-like | Cctz2 | TCP-1-zeta-like | Testis-specific protein TSA303 | Testis-specific Tcp20 | TSA303 | TCP-1-zeta-2 | CCT6B
Origin Species
Human
NCBI GenBank
NM_006584
SwissProt
Q92526

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAIKAVNSK AEVARARAAL AVNICAARGL QDVLRTNLGP KGTMKMLVSG AGDIKLTKDG
61NVLLDEMQIQ HPTASLIAKV ATAQDDVTGD GTTSNVLIIG ELLKQADLYI SEGLHPRIIA
121EGFEAAKIKA LEVLEEVKVT KEMKRKILLD VARTSLQTKV HAELADVLTE VVVDSVLAVR
181RPGYPIDLFM VEIMEMKHKL GTDTKEVNSG FFYKTAEEKE KLVKAERKFI EDRVQKIIDL
241KDKVCAQSNK GFVVINQKGI DPFSLDSLAK HGIVALRRAK RRNMERLSLA CGGMAVNSFE
301DLTVDCLGHA GLVYEYTLGE EKFTFIEECV NPCSVTLLVK GPNKHTLTQV KDAIRDGLRA
361IKNAIEDGCM VPGAGAIEVA MAEALVTYKN SIKGRARLGV QAFADALLII PKVLAQNAGY
421DPQETLVKVQ AEHVESKQLV GVDLNTGEPM VAADAGVWDN YCVKKQLLHS CTVIATNILL
481VDEIMRAGMS SLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.