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CCT6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCT6B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCT6B antibodies →
$504.00each
Catalog Number: LS-G115208-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCT6B (NM_006584), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCTZ-2 | CCT-zeta-2 | CCT-zeta-like | Cctz2 | TCP-1-zeta-like | Testis-specific protein TSA303 | Testis-specific Tcp20 | TSA303 | TCP-1-zeta-2 | CCT6B
Origin Species
Human
NCBI GenBank
NM_006584
SwissProt
Q92526

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAIKAVNSK AEVARAQAAL AVNICAARGL QDVLRTNLGP KGTMKMLASG AGDIKLTKDG
61NVLLDEMQIQ HPTASLIAKV ATAQDDVTGD GTTSNVLIIG ELLKQADLYI SEGLHPRIIA
121EGFEAAKIKA LEVLEEVKVT KEMKRKILLD VARTSLQTKV HAELADVLTE VVVDSVLAVR
181RPGYPIDLFM VEIMEMKHKL GTDTKLIQGL VLDHGARHPD MKKRVEDAFI LICNVSLEYE
241KTEVNSGFFY KTAEEKEKLV KAERKFIEDR VQKIIDLKDK VCAQSNKGFV VINQKGIDPF
301SLDSLAKHGI VALRRAKRRN MERLSLACGG MAVNSFEDLT VDCLGHAGLV YEYTLGEEKF
361TFIEECVNPC SVTLLVKGPN KHTLTQVKDA IRDGLRAIKN AIEDGCMVPG AGAIEVAMAE
421ALVTYKNSIK GRARLGVQAF ADALLIIPKV LAQNAGYDPQ ETLVKVQAEH VESKQLVGVD
481LNTGEPMVAA DAGVWDNYCV KKQLLHSCTV IATNILLVDE IMRAGMSSLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.