Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCR10 / GPR2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101457-100
Catalog Number: LS-G101457
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCR10(CCR10) (NM_016602), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-C CKR-10 | CC-CKR-10 | CCR-10 | C-C chemokine receptor type 10 | CC chemokine receptor 10 | CCR10 | G-protein coupled receptor 2 | GPR2 | G protein-coupled receptor 2
- Origin Species
- Human
- NCBI GenBank
- NM_016602
- SwissProt
- P46092
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTEATEQVS WGHYSGDEED AYSAEPLPEL CYKADVQAFS RAFQPSVSLT VAALGLAGNG61LVLATHLAAR RAARSPTSAH LLQLALADLL LALTLPFAAA GALQGWSLGS ATCRTISGLY121SASFHAGFLF LACISADRYV AIARALPAGP RPSTPGRAHL VSVIVWLLSL LLALPALLFS181QDGQREGQRR CRLIFPEGLT QTVKGASAVA QVALGFALPL GVMVACYALL GRTLLAARGP241ERRRALRVVV ALVAAFVVLQ LPYSLALLLD TADLLAARER SCPASKRKDV ALLVTSGLAL301ARCGLNPVLY AFLGLRFRQD LRRLLRGGSC PSGPQPRRGC PRRPRLSSCS APTETHSLSW361DNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
362 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.