Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCM2 / Malcavernin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129713-100
Catalog Number: LS-G129713
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCM2 (NM_001167934), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C7orf22 | CCM2 | Malcavernin | PP10187
- Origin Species
- Human
- NCBI GenBank
- NM_031443
- SwissProt
- Q9BSQ5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEEGKKGKK YLGQLTSIPG YLNPSSRTEI LHFIDNAKRA HQLPGHLTQE HDAVLSLSAY61NVKLAWRDGE DIILRVPIHD IAAVSYVRDD AAHLVVLKTA QDPGISPSQS LCAESSRGLS121AGSLSESAVG PVEACCLVIL AAESKVAAEE LCCLLGQVFQ VVYTESTIDF LDRAIFDGAS181TPTHHLSLHS DDSSTKVDIK ETYEVEASTF CFPESVDVGG ASPHSKTISE SELSASATEL241LQDYMLTLRT KLSSQEIQQF AALLHEYRNG ASIHEFCINL RQLYGDSRKF LLLGLRPFIP301EKDSQHFENF LETIGVKDGR GIITDSFGRH RRALSTTSSS TTNGNRATGS SDDRSAPSEG361DEWDRMISDI SSDIEALGCS MDQDSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
386 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.