Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CCM2 / Malcavernin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121011-100
Catalog Number: LS-G121011
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCM2 (NM_001029835), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C7orf22 | CCM2 | Malcavernin | PP10187
- Origin Species
- Human
- NCBI GenBank
- NM_031443
- SwissProt
- Q9BSQ5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MHSSCRQRRN QNLSKEIPQT EFHTGYSMEN EPGIVSPFKR VFLKGEKSRD KKAHEKVTER61RPLHTVVLSL PERVEPDRLL SDYIEKEVKY LGQLTSIPGY LNPSSRTEIL HFIDNAKRAH121QLPGHLTQEH DAVLSLSAYN VKLAWRDGED IILRVPIHDI AAVSYVRDDA AHLVVLKTAQ181DPGISPSQSL CAESSRGLSA GSLSESAVGP VEACCLVILA AESKVAAEEL CCLLGQVFQV241VYTESTIDFL DRAIFDGAST PTHHLSLHSD DSSTKVDIKE TYEVEASTFC FPESVDVGGA301SPHSKTISES ELSASATELL QDYMLTLRTK LSSQEIQQFA ALLHEYRNGA SIHEFCINLR361QLYGDSRKFL LLGLRPFIPE KDSQHFENFL ETIGVKDGRG IITDSFGRHR RALSTTSSST421TNGNRATGSS DDRSAPSEGD EWDRMISDIS SDIEALGCSM DQDSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
465 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.