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CCDC120 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC120 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC120 antibodies →
$504.00each
Catalog Number: LS-G128299-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC120 (NM_001163321), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
JM11 | CCDC120
Origin Species
Human
NCBI GenBank
AA133052

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVKGQLISS PTFNAPAALF GEAAPQVKSE RLRGLLDRQR TLQEALSLKL QELRKVCLQE
61AELTGQLPPE CPLEPGERPQ LVRRRPPTAR AYPPPHPNQA HHSLCPAEEL ALEALEREVS
121VQQQIAAAAR RLALAPDLST EQRRRRRQVQ ADALRRLHEL EEQLRDVRAR LGLPVLPLPQ
181PLPLSTGSVI TTQGVCLGMR LAQLSQEDVV LHSESSSLSE SGASHDNEEP HGCFSLAERP
241SPPKAWDQLR AVSGGSPERR TPWKPPPSDL YGDLKSRRNS VASPTSPTRS LPRSASSFEG
301RSVPATPVLT RGAGPQLCKP EGLHSRQWSG SQDSQMGFPR ADPASDRASL FVARTRRSNS
361SEALLVDRAA GGGAGSPPAP LAPSASGPPV CKSSEVLYER PQPTPAFSSR TAGPPDPPRA
421ARPSSAAPAS RGAPRLPPVC GDFLLDYSLD RGLPRSGGGT GWGELPPAAE VPGPLSRRDG
481LLTMLPGPPP VYAADSNSPL LRTKDPHTRA TRTKPCGLPP EAAEGPEVHP NPLLWMPPPT
541RIPSAGERSG HKNLALEGLR DWYIRNSGLA AGPQRRPVLP SVGPPHPPFL HARCYEVGQA
601LYGAPSQAPL PHSRSFTAPP VSGRYYADFL YPPELSARLS DLTLEGEQSS SSDTQTPGTL
661V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

661 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.