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CCDC120 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CCDC120 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CCDC120 antibodies →
$504.00each
Catalog Number: LS-G128296-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC120 (NM_001163322), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
JM11 | CCDC120
Origin Species
Human
NCBI GenBank
AA133052

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVKAALFGE AAPQVKSERL RGLLDRQRTL QEALSLKLQE LRKVCLQEAE LTGQLPPECP
61LEPGERPQLV RRRPPTARAY PPPHPNQAHH SLCPAEELAL EALEREVSVQ QQIAAAARRL
121ALAPDLSTEQ RRRRRQVQAD ALRRLHELEE QLRDVRARLG LPVLPLPQPL PLSTGSVITT
181QGVCLGMRLA QLSQEDVVLH SESSSLSESG ASHDNEEPHG CFSLAERPSP PKAWDQLRAV
241SGGSPERRTP WKPPPSDLYG DLKSRRNSVA SPTSPTRSLP RSASSFEGRS VPATPVLTRG
301AGPQLCKPEG LHSRQWSGSQ DSQMGFPRAD PASDRASLFV ARTRRSNSSE ALLVDRAAGG
361GAGSPPAPLA PSASGPPVCK SSEVLYERPQ PTPAFSSRTA GPPDPPRAAR PSSAAPASRG
421APRLPPVCGD FLLDYSLDRG LPRSGGGTGW GELPPAAEVP GPLSRRDGLL TMLPGPPPVY
481AADSNSPLLR TKDPHTRATR TKPCGLPPEA AEGPEVHPNP LLWMPPPTRI PSAGERSGHK
541NLALEGLRDW YIRNSGLAAG PQRRPVLPSV GPPHPPFLHA RCYEVGQALY GAPSQAPLPH
601SRSFTAPPVS GRYYADFLYP PELSARLSDL TLEGEQSSSS DTQTPGTLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

649 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.