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CBFA2T3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CBFA2T3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CBFA2T3 antibodies →
$725.00each
Catalog Number: LS-G116120-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CBFA2T3 (NM_005187), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ETO2 | MTG8-related gene 2 | MTGR2 | MTG16 | MTG8-related protein 2 | Protein CBFA2T3 | Translocated to | 3 | ZMYND4 | CBFA2T3 | HMTG16
Origin Species
Human
NCBI GenBank
NM_005187
SwissProt
O75081

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPASRLRDRA ASSASGSTCG SMSQTHPVLE SGLLASAGCS APRGPRKGGP APVDRKAKAS
61AMPDSPAEVK TQPRSTPPSM PPPPPAASQG ATRPPSFTPH THREDGPATL PHGRFHGCLK
121WSMVCLLMNG SSHSPTAING APCTPNGFSN GPATSSTASL STQHLPPACG ARQLSKLKRF
181LTTLQQFGSD ISPEIGERVR TLVLGLVNST LTIEEFHSKL QEATNFPLRP LVIPFLKANL
241PLLQRELLHC ARLAKQTPAQ YLAQHEQLLL DASASSPIDS SELLLEVNEN GKRRTPDRTK
301ENGSDRDPLH PEHLSKRPCT LNPAQRYSPS NGPPQPTPPP HYRLEDIAMA HHFRDAYRHP
361DPRELRERHR PLVVPGSRQE EVIDHKLTER EWAEEWKHLN NLLNCIMDMV EKTRRSLTVL
421RRCQEADREE LNHWARRYSD AEDTKKGPAP AAARPRSSSA GPEGPQLDVP REFLPRTLTG
481YVPEDIWRKA EEAVNEVKRQ AMSELQKAVS DAERKAHELI TTERAKMERA LAEAKRQASE
541DALTVINQQE DSSESCWNCG RKASETCSGC NAARYCGSFC QHRDWEKHHH VCGQSLQGPT
601AVVADPVPGP PEAAHSLGPS LPVGAASPSE AGSAGPSRPG SPSPPGPLDT VPR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

653 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.