Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CASP1 / Caspase 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108310-100
Catalog Number: LS-G108310
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caspase 1(CASP1) (NM_033293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Caspase-1 | CASP-1 | CASP1 | CASP1 nirs variant 1 | Caspase 1 | ICE | ICE-like protease | IL-1 beta-converting enzyme | IL-1BC | IL1BC | IL1BCE | p45 | IL1B-convertase | Interleukin-1 beta convertase
- Origin Species
- Human
- NCBI GenBank
- NM_001223
- SwissProt
- P29466
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADKVLKEKR KLFIRSMGEA PQAVQDNPAM PTSSGSEGNV KLCSLEEAQR IWKQKSAEIY61PIMDKSSRTR LALIICNEEF DSIPRRTGAE VDITGMTMLL QNLGYSVDVK KNLTASDMTT121ELEAFAHRPE HKTSDSTFLV FMSHGIREGI CGKKHSEQVP DILQLNAIFN MLNTKNCPSL181KDKPKVIIIQ ACRGDSPGVV WFKDSVGVSG NLSLPTTEEF EDDAIKKAHI EKDFIAFCSS241TPDNVSWRHP TMGSVFIGRL IEHMQEYACS CDVEEIFRKV RFSFEQPDGR AQMPTTERVT301LTRCFYLFPG HTRTRPLEQKLISEEDLAANDILDYKDDDDKV
311 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.