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CARD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CARD6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CARD6 antibodies →
$504.00each
Catalog Number: LS-G108717-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CARD6 (NM_032587), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CINCIN1 | CARD6
Origin Species
Human
NCBI GenBank
NM_032587
SwissProt
Q9BX69

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATESTPSEI IERERKKLLE ILQHDPDSIL DTLTSRRLIS EEEYETLENV TDLLKKSRKL
61LILVQKKGEA TCQHFLKCLF STFPQSAAIC GLRHEVLKHE NTVPPQSMGA SSNSEDAFSP
121GIKQPEAPEI TVFFSEKEHL DLETSEFFRD KKTSYRETAL SARKNEKEYD TPEVTLSYSV
181EKVGCEVPAT ITYIKDGQRY EELDDSLYLG KEEYLGSVDT PEDAEATVEE EVYDDPEHVG
241YDGEEDFENS ETTEFSGEEP SYEGSETSLS LEEEQEKSIE ERKKVFKDVL LCLNMDRSRK
301VLPDFVKQFS LDRGCKWTPE SPGDLAWNFL MKVQARDVTA RDSILSHKVL DEDSKEDLLA
361GVENLEIRDI QTINPLDVLC ATMLCSDSSL QRQVMSNMYQ CQFALPLLLP DAENNKSILM
421LGAMKDIVKK QSTQFSGGPT EDTEKFLTLM KMPVISFVRL GYCSFSKSRI LNTLLSPAQL
481KLHKIFLHQD LPLLVLPRQI SDGLVEITWC FPDSDDRKEN PFFQKPVALA NLRGNLESFW
541TQFGFLMEVS SAVFFFTDCL GEKEWDLLMF LGEAAIERCY FVLSSQARES EEAQIFQRIL
601NLKPAQLLFW ERGDAGDRRK NMEGLQAALQ EVMFSSCLRC VSVEDMAALA RELGIQVDED
661FENTQRIQVS SGENMAGTAE GEGQQRHSQL KSSSKSQALM PIQEPGTQCE LSQNLQNLYG
721TPVFRPVLEN SWLFPTRIGG NFNHVSLKAS WVMGRPFGSE QRPKWFHPLP FQNAGAQGRG
781KSFGIQSFHP QIFYSGERFM KFSRVARGCH SNGTFGRLPR PICQHVQACP ERPQMMGTLE
841RSRAVASKIG HSYSLDSQPA RAVGKPWPQQ ACTRVTELTE ATGKLIRTSH IGKPHPQSFQ
901PAAATQKLRP ASQQGVQMKT QGGASNPALQ IGSHPMCKSS QFKSDQSNPS TVKHSQPKPF
961HSVPSQPKSS QTKSCQSQPS QTKPSPCKST QPKPSQPWPP QSKPSQPRPP QPKSSSTNPS
1021QAKAHHSKAG QKRGGKH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1037 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.