Western blot analysis of extracts of various cell lines, using CARD6 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

CARD6 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human
Format:	Liquid

$281.00each

Catalog Number: LS-C748830-20

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Overview

Description: CARD6 antibody LS-C748830 is an unconjugated rabbit polyclonal antibody to human CARD6. Validated for WB.
Application: WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CINCIN1 | CARD6
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9BX69
NCBI GenBank: NM_032587 | NP_115976.2
SwissProt: Q9BX69

Target

Target: Human CARD6
Antigen Species: Human
Gene Name: CARD6

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CARD6 (NP_115976.2). MATESTPSEIIERERKKLLEILQHDPDSILDTLTSRRLISEEEYETLENVTDLLKKSRKLLILVQKKGEATCQHFLKCLFSTFPQSAAICGLRHEVLKHENTVPPQSMGASSNSEDAFSPGIKQPEAPEITVFFSEKEHLDLETSEFFRDKKTSYRETALSARKNEKEYDTPEVTLSYSVEKVGCEVPATITYIKDGQRY
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)