TIA-1 Antibody (clone TIA-1)
Catalog Number: LS-C312188-0.1
0.1 mL / $355.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Carboxylesterase 1 / CES1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121140-100
Catalog Number: LS-G121140
For bulk orders or custom formulations:
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Liver Carboxylesterase 1(CES1) (NM_001025195), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACAT | Brain carboxylesterase hBr1 | Carboxylesterase 1 | Carboxylesterase 2 (liver) | CEH | CES1 | CES1A1 | CES1A2 | Cocaine carboxylesterase | Egasyn | HMSE | Liver carboxylesterase 1 | HCE-1 | PCE-1 | Retinyl ester hydrolase | TGH | Triacylglycerol hydrolase | REH | SES1 | CE-1 | Cholesteryl ester hydrolase | HMSE1 | Serine esterase 1
- Origin Species
- Human
- NCBI GenBank
- L07764
- SwissProt
- P23141
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.59 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWLRAFILAT LSASAAWAGH PSSPPVVDTV HGKVLGKFVS LEGFAQPVAI FLGIPFAKPP61LGPLRFTPPQ PAEPWSFVKN ATSYPPMCTQ DPKAGQLLSE LFTNRKENIP LKLSEDCLYL121NIYTPADLTK KNRLPVMVWI HGGGLMVGAA STYDGLALAA HENVVVVTIQ YRLGIWGFFS181TGDEHSRGNW GHLDQVAALR WVQDNIASFG GNPGSVTIFG ESAGGESVSV LVLSPLAKNL241FHRAISESGV ALTSVLVKKG DVKPLAEQIA ITAGCKTTTS AVMVHCLRQK TEEELLETTL301KMKFLSLDLQ GDPRESQPLL GTVIDGMLLL KTPEELQAER NFHTVPYMVG INKQEFGWLI361PMQLMSYPLS EGQLDQKTAM SLLWKSYPLV CIAKELIPEA TEKYLGGTDD TVKKKDLFLD421LIADVMFGVP SVIVARNHRD AGAPTYMYEF QYRPSFSSDM KPKTVIGDHG DELFSVFGAP481FLKEGASEEE IRLSKMVMKF WANFARNGNP NGEGLPHWPE YNQKEGYLQI GANTQAAQKL541KDKEVAFWTN LFAKKAVEKP PQTEHIELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
568 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
