LSBio
Carboxylesterase 1 / CES1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Carboxylesterase 1 / CES1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CES1 antibodies →
$504.00each
Catalog Number: LS-G121139-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Liver Carboxylesterase 1(CES1) (NM_001025194), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACAT | Brain carboxylesterase hBr1 | Carboxylesterase 1 | Carboxylesterase 2 (liver) | CEH | CES1 | CES1A1 | CES1A2 | Cocaine carboxylesterase | Egasyn | HMSE | Liver carboxylesterase 1 | HCE-1 | PCE-1 | Retinyl ester hydrolase | TGH | Triacylglycerol hydrolase | REH | SES1 | CE-1 | Cholesteryl ester hydrolase | HMSE1 | Serine esterase 1
Origin Species
Human
NCBI GenBank
L07764
SwissProt
P23141

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.27 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWLPALVLAT LAASAAWGHP SSPPVVDTVH GKVLGKFVSL EGFAQPVAIF LGIPFAKPPL
61GPLRFTPPQP AEPWSFVKNA TSYPPMCTQD PKAGQLLSEL FTNRKENIPL KLSEDCLYLN
121IYTPADLTKK NRLPVMVWIH GGGLMVGAAS TYDGLALAAH ENVVVVTIQY RLGIWGFFST
181GDEHSRGNWG HLDQVAALRW VQDNIASFGG NPGSVTIFGE SAGGESVSVL VLSPLAKNLF
241HRAISESGVA LTSVLVKKGD VKPLAEQIAI TAGCKTTTSA VMVHCLRQKT EEELLETTLK
301MKFLSLDLQG DPRESQPLLG TVIDGMLLLK TPEELQAERN FHTVPYMVGI NKQEFGWLIP
361MLMSYPLSEG QLDQKTAMSL LWKSYPLVCI AKELIPEATE KYLGGTDDTV KKKDLFLDLI
421ADVMFGVPSV IVARNHRDAG APTYMYEFQY RPSFSSDMKP KTVIGDHGDE LFSVFGAPFL
481KEGASEEEIR LSKMVMKFWA NFARNGNPNG EGLPHWPEYN QKEGYLQIGA NTQAAQKLKD
541KEVAFWTNLF AKKAVEKPPQ TEHIELTRTR PLEQKLISEE DLAANDILDY KDDDDKV

597 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.