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CACNB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CACNB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103250-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACNB2 (NM_201593), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CACNB2 | Cavbeta2 | Cchb2 | CACNLB2 | MYSB | CAB2 | CAVB2
Origin Species
Human
NCBI GenBank
NM_000724
SwissProt
Q08289

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVQRDMSKSP PTAAAAVAQE IQMELLENVA PAGALGAAAQ SYGKGARRKN RFKGSDGSTS
61SDTTSNSFVR QGSADSYTSR PSDSDVSLEE DREAVRREAE RQAQAQLEKA KTKPVAFAVR
121TNVSYSAAHE DDVPVPGMAI SFEAKDFLHV KEKFNNDWWI GRLVKEGCEI GFIPSPVKLE
181NMRLQHEQRA KQGKFYSSKS GGNSSSSLGD IVPSSRKSTP PSSAKQKQKS TEHTPPYDVV
241PSMRPVVLVG PSLKGYEVTD MMQKALFDFL KHRFEGRISI TRVTADISLA KRSVLNNPSK
301HAIIERSNTR SSLAEVQSEI ERIFELARTL QLVVLDADTI NHPAQLSKTS LAPIIVYVKI
361SSPKVLQRLI KSRGKSQAKH LNVQMVAADK LAQCPPELFD VILDENQLED ACEHLADYLE
421AYWKATHPPS SSLPNPLLSR TLATSSLPLS PTLASNSQGS QGDQRTDRSA PIRSASQAEE
481EPSVEPVKKS QHRSSSSAPH HNHRSGTSRG LSRQETFDSE TQESRDSAYV EPKEDYSHDH
541VDHYASHRDH NHRDETHGSS DHRHRESRHR SRDVDREQDH NECNKQRSRH KSKDRYCEKD
601GEVISKKRNE AGEWNRDVYI RQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

622 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.