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CACNB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CACNB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103247-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACNB2 (NM_201590), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CACNB2 | Cavbeta2 | Cchb2 | CACNLB2 | MYSB | CAB2 | CAVB2
Origin Species
Human
NCBI GenBank
NM_000724
SwissProt
Q08289

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68 kda
Protein Type
Lysate
Amino Acid Sequence
1MLDRRLIAPQ TKYIIPGGSA DSYTSRPSDS DVSLEEDREA VRREAERQAQ AQLEKAKTKP
61VAFAVRTNVS YSAAHEDDVP VPGMAISFEA KDFLHVKEKF NNDWWIGRLV KEGCEIGFIP
121SPVKLENMRL QHEQRAKQGK FYSSKSGGNS SSSLGDIVPS SRKSTPPSSA IDIDATGLDA
181EENDIPANHR SPKPSANSVT SPHSKEKRMP FFKKTEHTPP YDVVPSMRPV VLVGPSLKGY
241EVTDMMQKAL FDFLKHRFEG RISITRVTAD ISLAKRSVLN NPSKHAIIER SNTRSSLAEV
301QSEIERIFEL ARTLQLVVLD ADTINHPAQL SKTSLAPIIV YVKISSPKVL QRLIKSRGKS
361QAKHLNVQMV AADKLAQCPP ELFDVILDEN QLEDACEHLA DYLEAYWKAT HPPSSSLPNP
421LLSRTLATSS LPLSPTLASN SQGSQGDQRT DRSAPIRSAS QAEEEPSVEP VKKSQHRSSS
481SAPHHNHRSG TSRGLSRQET FDSETQESRD SAYVEPKEDY SHDHVDHYAS HRDHNHRDET
541HGSSDHRHRE SRHRSRDVDR EQDHNECNKQ RSRHKSKDRY CEKDGEVISK KRNEAGEWNR
601DVYIRQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

606 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.