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BTBD9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTBD9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119019-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD9 (NM_001099272), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTB (POZ) domain containing 9 | DJ322I12.1 | KIAA1880 | BTBD9
Origin Species
Human
NCBI GenBank
NM_001099272
SwissProt
Q96Q07

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSNSHPLRPF TAVGEIDHVH ILSEHIGALL IGEEYGDVTF VVEKKRFPAH RVILAARCQY
61FRALLYGGMR ESQPEAEIPL QDTTAEAFTM LLKYIYTGRA TLTDEKEEVL LDFLSLAHKY
121GFPELEDSTS EYLCTILNIQ NVCMTFDVAS LYSLPKLTCM CCMFMDRNAQ EVLSSEGFLS
181LSKTALLNIV LRDSFAAPEK DIFLALLNWC KHNSKENHAE IMQAVRLPLM SLTELLNVVR
241PSGLLSPDAI LDAIKVRSES RDMDLNYRGM LIPEENIATM KYGAQVVKGE LKSALLDGDT
301QNYDLDHGFS RHPIDDDCRS GIEIKLGQPS IINHIRILLW DRDSRSYSYF IEVSMDELDW
361VRVIDHSQYL CRSWQKLYFP ARVCRYIRIV GTHNTVNKIF HIVAFECMFT NKTFTLEKGL
421IVPMENVATI ADCASVIEGV SRSRNALLNG DTKNYDWDSG YTCHQLGSGA IVVQLAQPYM
481IGSIRLLLWD CDDRSYSYYV EVSTNQQQWT MVADRTKVSC KSWQSVTFER QPASFIRIVG
541THNTANEVFH CVHFECPEQQ SSQKEENSEE SGTGDTSLAG QQLDSHALRA PSGSSLPSSP
601GSNSRSPNRQ HQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

612 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.