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BTBD9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BTBD9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all BTBD9 antibodies →
$725.00each
Catalog Number: LS-G106058-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD9 (NM_152733), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BTB (POZ) domain containing 9 | DJ322I12.1 | KIAA1880 | BTBD9
Origin Species
Human
NCBI GenBank
NM_001099272
SwissProt
Q96Q07

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRESQPEAEI PLQDTTAEAF TMLLKYIYTG RATLTDEKEE VLLDFLSLAH KYGFPELEDS
61TSEYLCTILN IQNVCMTFDV ASLYSLPKLT CMCCMFMDRN AQEVLSSEGF LSLSKTALLN
121IVLRDSFAAP EKDIFLALLN WCKHNSKENH AEIMQAVRLP LMSLTELLNV VRPSGLLSPD
181AILDAIKVRS ESRDMDLNYR GMLIPEENIA TMKYGAQVVK GELKSALLDG DTQNYDLDHG
241FSRHPIDDDC RSGIEIKLGQ PSVINHIRIL LWDRDSRSYS YFIEVSMDEL DWVRVIDHSQ
301YLCRSWQKLY FPARVCRYIR IVGTHNTVNK IFHIVAFECM FTNKTFTLEK GLIVPMENVA
361TIADCASVIE GVSRSRNALL NGDTKNYDWD SGYTCHQLGS GAIVVQLAQP YMIGSIRLLL
421WDCDDRSYSY YVEVSTNQQQ WTMVADRTKV SCKSWQSVTF ERQPASFIRI VGTHNTANEV
481FHCVHFECPE QQSSQKEENS EESGTGDTSL AGQQLDSHAL RAPSGSSLPS SPGSNSRSPN
541RQHQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

544 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.