Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BAF60C / SMARCD3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122500-100
Catalog Number: LS-G122500
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMARCD3 (NM_001003802), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRG1-associated factor 60C | Rsc6p | Swp73-like protein | SMARCD3 | BAF60C | CRACD3
- Origin Species
- Human
- NCBI GenBank
- NM_001003801
- SwissProt
- Q6STE5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTPGLQHPPT VVQRPGMPSG ARMPHQGAPM GPPGSPYMGS PAVRPGLAPA GMEPARKRAA61PPPGQSQAQS QGQPVPTAPA RSRSAKRRKM ADKILPQRIR ELVPESQAYM DLLAFERKLD121QTIMRKRVDI QEALKRPMKQ KRKLRLYISN TFNPAKPDAE DSDGSIASWE LRVEGKLLDD181PSKQKRKFSS FFKSLVIELD KDLYGPDNHL VEWHRTPTTQ ETDGFQVKRP GDLSVRCTLL241LMLDYQPPQF KLDPRLARLL GLHTQSRSAI VQALWQYVKT NRLQDSHDKE YINGDKYFQQ301IFDCPRLKFS EIPQRLTALL LPPDPIVINH VISVDPSDQK KTACYDIDVE VEEPLKGQMS361SFLLSTANQQ EISALDSKIH ETIESINQLK IQRDFMLSFS RDPKGYVQDL LRSQSRDLKV421MTDVAGNPEE ERRAEFYHQP WSQEAVSRYF YCKIQQRRQE LEQSLVVRNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
470 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.