Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
BAF60C / SMARCD3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122499-100
Catalog Number: LS-G122499
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMARCD3 (NM_001003801), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRG1-associated factor 60C | Rsc6p | Swp73-like protein | SMARCD3 | BAF60C | CRACD3
- Origin Species
- Human
- NCBI GenBank
- NM_001003801
- SwissProt
- Q6STE5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAADEVAGGA RKATKSKLFE FLVHGVRPGM PSGARMPHQG APMGPPGSPY MGSPAVRPGL61APAGMEPARK RAAPPPGQSQ AQSQGQPVPT APARSRSAKR RKMADKILPQ RIRELVPESQ121AYMDLLAFER KLDQTIMRKR VDIQEALKRP MKQKRKLRLY ISNTFNPAKP DAEDSDGSIA181SWELRVEGKL LDDPSKQKRK FSSFFKSLVI ELDKDLYGPD NHLVEWHRTP TTQETDGFQV241KRPGDLSVRC TLLLMLDYQP PQFKLDPRLA RLLGLHTQSR SAIVQALWQY VKTNRLQDSH301DKEYINGDKY FQQIFDCPRL KFSEIPQRLT ALLLPPDPIV INHVISVDPS DQKKTACYDI361DVEVEEPLKG QMSSFLLSTA NQQEISALDS KIHETIESIN QLKIQRDFML SFSRDPKGYV421QDLLRSQSRD LKVMTDVAGN PEEERRAEFY HQPWSQEAVS RYFYCKIQQR RQELEQSLVV481RNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
483 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.