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BACH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

BACH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101762-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BACH2 (NM_021813), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BACH2 | BTBD25 | BTB and CNC homolog 2
Origin Species
Human
NCBI GenBank
NM_021813
SwissProt
Q9BYV9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVDEKPDSP MYVYESTVHC TNILLGLNDQ RKKDILCDVT LIVERKEFRA HRAVLAACSE
61YFWQALVGQT KNDLVVSLPE EVTARGFGPL LQFAYTAKLL LSRENIREVI RCAEFLRMHN
121LEDSCFSFLQ TQLLNSEDGL FVCRKDAACQ RPHEDCENSA GEEEDEEEET MDSETAKMAC
181PRDQMLPEPI SFEAAAIPVA EKEEALLPEP DVPTDTKESS EKDALTQYPR YKKYQLACTK
241NVYNASSHST SGFASTFRED NSSNSLKPGL ARGQIKSEPP SEENEEESIT LCLSGDEPDA
301KDRAGDVEMD RKQPSPAPTP TAPAGAACLE RSRSVASPSC LRSLFSITKS VELSGLPSTS
361QQHFARSPAC PFDKGITQGD LKTDYTPFTG NYGQPHVGQK EVSNFTMGSP LRGPGLEALC
421KQEGELDRRS VIFSSSACDQ VSTSVHSYSG VSSLDKDLSE PVPKGLWVGA GQSLPSSQAY
481SHGGLMADHL PGRMRPNTSC PVPIKVCPRS PPLETRTRTS SSCSSYSYAE DGSGGSPCSL
541PLCEFSSSPC SQGARFLATE HQEPGLMGDG MYNQVRPQIK CEQSYGTNSS DESGSFSEAD
601SESCPVQDRG QEVKLPFPVD QITDLPRNDF QMMIKMHKLT SEQLEFIHDV RRRSKNRIAA
661QRCRKRKLDC IQNLECEIRK LVCEKEKLLS ERNQLKACMG ELLDNFSCLS QEVCRDIQSP
721EQIQALHRYC PVLRPMDLPT ASSINPAPLG AEQNIAASQC AVGENVPCCL EPGAAPPGPP
781WAPSNTSENC TSGRRLEGTD PGTFSERGPP LEPRSQTVTV DFCQEMTDKC TTDEQPRKDY
841T
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

841 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.