Western Blot of rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 µg per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted (native)/Observed (over-expressed) size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none.

BACH2 Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Format:	Liquid

$311.00each

Catalog Number: LS-C745432-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: BACH2 antibody LS-C745432 is an unconjugated rabbit polyclonal antibody to mouse BACH2 (N-Terminus). Validated for ELISA, IP and WB.
Application: ELISA, IP, WB
Predicted Reactivity: Mouse
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BACH2 | BTBD25 | BTB and CNC homolog 2
Recommended Dilution: ELISA: 1:5000
Recommended Dilution: IP: 1 µg/ml
Recommended Dilution: WB: 1 µg/ml
UniProt Number: Q9BYV9
NCBI GenBank: NM_021813 | NP_068585.1
SwissProt: Q9BYV9

Target

Target: Mouse BACH2
Antigen Species: Mouse
Epitope: A BLAST analysis was used to suggest 100% homology with Bach2 protein from mouse. Reactivity against homologues from other sources is not known.
Gene Name: BACH2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.97 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl