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B3GNT5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

B3GNT5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109055-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human B3GNT5 (NM_032047), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B3GNT5 | Beta-1 | 3-Gn-T5 | Lc(3)Cer synthase | Lc3 synthase | B3GN-T5 | Beta3Gn-T5 | BGnT-5 | Lactotriaosylceramide synthase
Origin Species
Human
NCBI GenBank
NM_032047
SwissProt
Q9BYG0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRMLVSGRRV KKWQLIIQLF ATCFLASLMF FWEPIDNHIV SHMKSYSYRY LINSYDFVND
61TLSLKHTSAG PRYQYLINHK EKCQAQDVLL LLFVKTAPEN YDRRSGIRRT WGNENYVRSQ
121LNANIKTLFA LGTPNPLEGE ELQRKLAWED QRYNDIIQQD FVDSFYNLTL KLLMQFSWAN
181TYCPHAKFLM TADDDIFIHM PNLIEYLQSL EQIGVQDFWI GRVHRGAPPI RDKSSKYYVS
241YEMYQWPAYP DYTAGAAYVI SGDVAAKVYE ASQTLNSSLY IDDVFMGLCA NKIGIVPQDH
301VFFSGEGKTP YHPCIYEKMM TSHGHLEDLQ DLWKNATDPK VKTISKGFFG QIYCRLMKII
361LLCKISYVDT YPCRAAFI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

378 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.