Western blot analysis of extracts of various cell lines, using B3GNT5 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

B3GNT5 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C749430-20

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Overview

Description: B3GNT5 antibody LS-C749430 is an unconjugated rabbit polyclonal antibody to B3GNT5 from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: B3GNT5 | Beta-1,3-Gn-T5 | Lc(3)Cer synthase | Lc3 synthase | B3GN-T5 | Beta3Gn-T5 | BGnT-5 | Lactotriaosylceramide synthase
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9BYG0
NCBI GenBank: NM_032047 | NP_114436.1
SwissProt: Q9BYG0

Target

Target: Human B3GNT5
Antigen Species: Human
Gene Name: B3GNT5

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_114436.1). DNHIVSHMKSYSYRYLINSYDFVNDTLSLKHTSAGPRYQYLINHKEKCQAQDVLLLLFVKTAPENYDRRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGEELQRKLAWEDQRYNDIIQQDFVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSLEQIGVQDFWIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKVKTISKGFFGQIYCRLMKIILLCKISYVDTYPCRAAFI
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)