Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARNTL / BMAL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99358-100
Catalog Number: LS-G99358
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BMAL1(ARNTL) (NM_001178), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BHLH-PAS protein JAP3 | ARNTL | BHLHe5 | BMAL1c | JAP3 | PASD3 | Member of PAS protein 3 | MOP3 | TIC | Brain and muscle ARNT-like 1 | Member of PAS superfamily 3
- NCBI GenBank
- NM_001178
- SwissProt
- O00327
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADQRMDISS TISDFMSPGP TDLLSSSLGT SGVDCNRKRK GSSTDYQESM DTDKDDPHGR61LEYTEHQGRI KNAREAHSQI EKRRRDKMNS FIDELASLVP TCNAMSRKLD KLTVLRMAVQ121HMKTLRGATN PYTEANYKPT FLSDDELKHL ILRAADGFLF VVGCDRGKIL FVSESVFKIL181NYSQNDLIGQ SLFDYLHPKD IAKVKEQLSS SDTAPRERLI DAKTGLPVKT DITPGPSRLC241SGARRSFFCR MKCNRPSVKV EDKDFPSTCS KKKDRKSFCT IHSTGYLKSW PPTKMGLDED301NEPDNEGCNL SCLVAIGRLH SHVVPQPVNG EIRVKSMEYV SRHAIDGKFV FVDQRATAIL361AYLPQELLGT SCYEYFHQDD IGHLAECHRQ VLQTREKITT NCYKFKIKDG SFITLRSRWF421SFMNPWTKEV EYIVSTNTVV LANVLEGGDP TFPQLTASPH SMDSMLPSGE GGPKRTHPTV481PGIPGGTRAG AGKIGRMIAE EIMEIHRIRG SSPSSCGSSP LNITSTPPPD ASSPGGKKIL541NGGTPDIPSS GLLSGQAQEN PGYPYSDSSS ILGENPHIGI DMIDNDQGSS SPSNDEAAMA601VIMSLLEADA GLGGPVDFSD LPWPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
625 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.