Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARNTL / BMAL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120795-100
Catalog Number: LS-G120795
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BMAL1(ARNTL) (NM_001030273), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BHLH-PAS protein JAP3 | ARNTL | BHLHe5 | BMAL1c | JAP3 | PASD3 | Member of PAS protein 3 | MOP3 | TIC | Brain and muscle ARNT-like 1 | Member of PAS superfamily 3
- Origin Species
- Human
- NCBI GenBank
- NM_001178
- SwissProt
- O00327
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MINIESMDTD KDDPHGRLEY TEHQGRIKNA REAHSQIEKR RRDKMNSFID ELASLVPTCN61AMSRKLDKLT VLRMAVQHMK TLRGATNPYT EANYKPTFLS DDELKHLILR AADGFLFVVG121CDRGKILFVS ESVFKILNYS QNDLIGQSLF DYLHPKDIAK VKEQLSSSDT APRERLIDAK181TGLPVKTDIT PGPSRLCSGA RRSFFCRMKC NRPSVKVEDK DFPSTCSKKK ADRKSFCTIH241STGYLKSWPP TKMGLDEDNE PDNEGCNLSC LVAIGRLHSH VVPQPVNGEI RVKSMEYVSR301HAIDGKFVFV DQRATAILAY LPQELLGTSC YEYFHQDDIG HLAECHRQVL QTREKITTNC361YKFKIKDGSF ITLRSRWFSF MNPWTKEVEY IVSTNTVVLA NVLEGGDPTF PQLTASPHSM421DSMLPSGEGG PKRTHPTVPG IPGGTRAGAG KIGRMIAEEI MEIHRIRGSS PSSCGSSPLN481ITSTPPPDAS SPGGKKILNG GTPDIPSSGL LSGQAQENPG YPYSDSSSIL GENPHIGIDM541IDNDQGSSSP SNDEAAMAVI MSLLEADAGL GGPVDFSDLP WPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
583 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.