Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ARHGAP22 / RhoGAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110688-100
Catalog Number: LS-G110688
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGAP22 (NM_021226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGAP22 | RhoGAP2 | RhoGap22
- Origin Species
- Human
- NCBI GenBank
- NM_021226
- SwissProt
- Q7Z5H3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSPKIRQAR RARSKSLVMG EQSRSPGRMP CPHRLGPVLK AGWLKKQRSI MKNWQQRWFV61LRGDQLFYYK DKDEIKPQGF ISLQGTQVTE LPPGPEDPGK HLFEISPGGA GEREKVPANP121EALLLMASSQ RDMEDWVQAI RRVIWAPLGG GIFGQRLEET VHHERKYGPR LAPLLVEQCV181DFIRERGLTE EGLFRMPGQA NLVRDLQDSF DCGEKPLFDS TTDVHTVASL LKLYLRELPE241PVVPFARYED FLSCAQLLTK DEGEGTLELA KQVSNLPQAN YNLLRYICKF LDEVQAYSNV301NKMSVQNLAT VFGPNILRPQ VEDPVTIMEG TSLVQHLMTV LIRKHSQLFT APVPEGPTSP361RGGLQCAVGW GSEEVTRDSQ GEPGGPGLPA HRTSSLDGAA VAVLSRTAPT GPGSRCSPGK421KVQTLPSWKS SFRQPRSLSG SPKGGGSSLE VPIISSGGNW LMNGLSSLRG HRRASSGDRL481KDSGSVQRLS TYDNVPAPGL VPGIPSVASM AWSGASSSES SVGGSLSSCT ACRASDSSAR541SSLHTDWALE PSPLPSSSED PKSLDLDHSM DEAGAGASNS EPSEPDSPTR EHARRSEALQ601GLVTELRAEL CRQRTEYERS VKRIEEGSAD LRKRMSRLEE ELDQEKKKYI MLEIKLRNSE661RAREDAERRN QLLQREMEEF FSTLGSLTVG AKGARAPKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
698 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.