Western Blot of Rabbit anti-ARHGAP22 pS22 antibody. Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutaion. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800® rabbit secondary antibody at 1:10,000 for 45 min at RT.
ARHGAP22 / RhoGAP2 Antibody (phospho-Ser22)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209346-50
Catalog Number: LS-C209346
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- Description
- RhoGAP2 antibody LS-C209346 is an unconjugated rabbit polyclonal antibody to RhoGAP2 (ARHGAP22) (pSer22) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARHGAP22 | RhoGAP2 | RhoGap22
- Recommended Dilution: ELISA
- 1:20000 - 1:60000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:100 - 1:500
- UniProt Number
- Q7Z5H3
- NCBI GenBank
- NM_021226 | NP_067049.2
- SwissProt
- Q7Z5H3
Target
- Target
- Mouse ARHGAP22 / RhoGAP2
- Antigen Species
- Mouse
- Epitope
- This antibody is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397->A mutation but not the S22->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS22 from other sources has not been determined.
- Gene Name
- ARHGAP22
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 50% Glycerol
- Concentration
- 1.07 mg/mL
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl