Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
APBB1 / FE65 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106613-100
Catalog Number: LS-G106613
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FE65(APBB1) (NM_145689), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APBB1 | FE65 | Adaptor protein FE65a2 | Protein Fe65 | Stat-like protein | MGC:9072 | RIR
- Origin Species
- Human
- NCBI GenBank
- NM_001164
- SwissProt
- O00213
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVPSSLSQS AINANSHGGP ALSLPLPLHA AHNQLLNAKL QATAVGPKDL RSAMGEGGGP61EPGPANAKWL KEGQNQLRRA ATAHRDQNRN VTLTLAEEAS QEPEMAPLGP KGLIHLYSEL121ELSAHNAANR GLRGPGLIIS TQEQGPDEGE EKAAGEAEEE EEDDDDEEEE EDLSSPPGLP181EPLESVEAPP RPQALTDGPR EHSKSASLLF GMRNSAASDE DSSWATLSQG SPSYGSPEDT241DSFWNPNAFE TDSDLPAGWM RVQDTSGTYY WHIPTGTTQW EPPGRASPSQ GSSPQEESQL301TWTGFAHGEG FEDGEFWKDE PSDEAPMELG LKEPEEGTLT FPAQSLSPEP LPQEEEKLPP361RNTNPGIKCF AVRSLGWVEM TEEELAPGRS SVAVNNCIRQ LSYHKNNLHD PMSGGWGEGK421DLLLQLEDET LKLVEPQSQA LLHAQPIISI RVWGVGRDSG RDFAYVARDK LTQMLKCHVF481RCEAPAKNIA TSLHEICSKI MAERRNARCL VNGLSLDHSK LVDVPFQVEF PAPKNELVQK541FQVYYLGNVP VAKPVGVDVI NGALESVLSS SSREQWTPSH VSVAPATLTI LHQQTEAVLG601ECRVRFLSFL AVGRDVHTFA FIMAAGPASF CCHMFWCEPN AASLSEAVQA ACMLRYQKCL661DARSQASTSC LPAPPAESVA RRVGWTVRRG VQSLWGSLKP KRLGAHTPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
708 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.