Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA13 / Annexin XIII Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122527-100
Catalog Number: LS-G122527
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin A13(ANXA13) (NM_001003954), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Annexin XIII | Annexin-13 | ANX13 | ANXA13 | Intestine-specific annexin | ISA | Annexin A13 | Annexin | intestine-specific
- Origin Species
- Human
- NCBI GenBank
- NM_004306
- SwissProt
- P27216
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNRHSQSYT LSEGSQQLPK GDSQPSTVVQ PLSHPSRNGE PEAPQPAKAS SPQGFDVDRD61AKKLNKACKG MGTNEAAIIE ILSGRTSDER QQIKQKYKAT YGKELEEVLK SELSGNFEKT121ALALLDRPSE YAARQLQKAM KGLGTDESVL IEVLCTRTNK EIIAIKEAYQ RLFDRSLESD181VKGDTSGNLK KILVSLLQAN RNEGDDVDKD LAGQDAKDLY DAGEGRWGTD ELAFNEVLAK241RSYKQLRATF QAYQILIGKD IEEAIEEETS GDLQKAYLTL VRCAQDCEDY FAERLYKSMK301GAGTDEETLI RIVVTRAEVD LQGIKAKFQE KYQKSLSDMV RSDTSGDFRK LLVALLHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
357 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.