Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA13 / Annexin XIII Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116732-100
Catalog Number: LS-G116732
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin A13(ANXA13) (NM_004306), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Annexin XIII | Annexin-13 | ANX13 | ANXA13 | Intestine-specific annexin | ISA | Annexin A13 | Annexin | intestine-specific
- Origin Species
- Human
- NCBI GenBank
- NM_004306
- SwissProt
- P27216
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNRHAKASS PQGFDVDRDA KKLNKACKGM GTNEAAIIEI LSGRTSDERQ QIKQKYKATY61GKELEEVLKS ELSGNFEKTA LALLDHPSEY AARQLQKAMK GLGTDESVLI EVLCTRTNKE121IIAIKEAYQR LFDRSLESDV KGDTSGNLKK ILVSLLQANR NEGDDVDKDL AGQDAKDLYD181AGEGRWGTDE LAFNEVLAKR SYKQLRATFQ AYQILIGKDI EEAIEEETSG DLQKAYLTLV241RCAQDCEDYF AERLYKSMKG AGTDEETLIR IIVTRAEVDL QGIKAKFQEK YQKSLSDMVR301SDTSGDFRKL LVALLHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
316 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.