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ANKS1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKS1B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKS1B antibodies →
$725.00each
Catalog Number: LS-G106007-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKS1B (NM_152788), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIDA-1 | ANKS1B | Cajalin-2 | Cajalin 2 | EB1 | E2A-PBX1-associated protein | EB-1 | ANKS2
Origin Species
Human
NCBI GenBank
NM_020140
SwissProt
Q7Z6G8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
137.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKDQELLEA ARTGNVALVE KLLSGRKGGI LGGGSGPLPL SNLLSIWRGP NVNCTDSSGY
61TALHHAALNG HKDIVLKLLQ YEASTNVADN KGYFPIHLAA WKGDVEIVKI LIHHGPSHSR
121VNEQNNENET ALHCAAQYGH SEVVAVLLEE LTDPTIRNSK LETPLDLAAL YGRLRVVKMI
181ISAHPNLMSC NTRKHTPLHL AARNGHKAVV QVLLEAGMDV SCQTEKGSAL HEAALFGKVD
241VVRVLLETGI DANIKDSLGR TVLDILKEHP SQKSLQIATL LQEYLEGVGR STVLEEPVQE
301DATQETHISS PVESPSQKTK SETVTGELSK LLDEIKLCQE KDYSFEDLCH TISDHYLDNL
361SKISEEELGK NGSQSVRTSS TINLSPGEVE EEDDDENTCG PSGLWEALTP CNGCRNLGFP
421MLAQESYPKK RNYTMEIVPS ASLDTFPSEN ENFLCDLMDT AVTKKPCSLE IARAPSPRTD
481NASEVAVTTP GTSNHRNSST GPTPDCSPPS PDTALKNIVK VIRPQPKQRT SIVSSLDFHR
541MNHNQEYFEI NTSTGCTSFT ASPPASPPTS SVGTTEVKNE GTNHTDDLSR QDDNDPPKEY
601DPGQFAGLLH GSSPACESPE NPFHLYGKRE QCEKGQDEVS LANSPLPFKQ SPIENNSEPL
661VKKIKPKVVS RTIFHKKSNQ LENHTIVGTR STRSGSRNGD QWVMNAGGFV ERACTLGRIR
721SLPKALIDMH LSKSVSKSDS DLIAYPSNEK TSRVNWSESS TAEHSSKGNS ERTPSFTSEW
781EEIDKIMSSI DVGINNELKE MNGETTRPRC PVQTVGQWLE SIGLPQYENH LMANGFDNVQ
841FMGSNVMEDQ DLLEIGILNS GHRQRILQAI QLLPKMRPIG HDGYHPTSVA EWLDSIELGD
901YTKAFLINGY TSMDLLKKIW EVELINVLKI NLIGHRKRIL ASLGDRLHDD PPQKPPRSIT
961LREPSGNHTP PQLSPSLSQS TYTTGGSLDV PHIIMQGDAR RRRNENYFDD IPRSKLERQM
1021AQTGDWGEPS ITLRPPNEAT ASTPVQYWQH HPEKLIFQSC DYKAFYLGSM LIKELRGTES
1081TQDACAKMRA NCQKSTEQMK KVPTIILSVS YKGVKFIDAT NKNIIAEHEI RNISCAAQDP
1141EDLSTFAYIT KDLKSNHHYC HVFTAFDVNL AYEIILTLGQ AFEVAYQLAL QARKGGHSST
1201LPESFENKPS KPIPKPRVSI RKSVDLLHAS HTGQEPSERH TEEALRKF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1248 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.