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ANKS1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKS1B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKS1B antibodies →
$725.00each
Catalog Number: LS-G104316-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKS1B (NM_181670), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIDA-1 | ANKS1B | Cajalin-2 | Cajalin 2 | EB1 | E2A-PBX1-associated protein | EB-1 | ANKS2
Origin Species
Human
NCBI GenBank
NM_020140
SwissProt
Q7Z6G8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMWQCHLSAQ DYRYYPVDGY SLLKRFPLHP LTGPRCPVQT VGQWLESIGL PQYENHLMAN
61GFDNVQFMGS NVMEDQDLLE IGILNSGHRQ RILQAIQLLP KMRPIGHDGY HPTSVAEWLD
121SIELGDYTKA FLINGYTSMD LLKKIWEVEL INVLKINLIG HRKRILASLG DRLHDDPPQK
181PPRSITLREP SGNHTPPQLS PSLSQSTYTT GGSLDVPHII MQGDARRRRN ENYFDDIPRS
241KLERQMAQSS VCEIWTNQNA GFPFSAIHQV HNTGDWGEPS ITLRPPNEAT ASTPVQYWQH
301HPEKLIFQSC DYKAFYLGSM LIKELRGTES TQDACAKMRA NCQKSTEQMK KVPTIILSVS
361YKGVKFIDAT NKNIIAEHEI RNISCAAQDP EDLSTFAYIT KDLKSNHHYC HVFTAFDVNL
421AYEIILTLGQ AFEVAYQLAL QARKGGHSST LPESFENKPS KPIPKPRVSI RKSVQIDPSE
481QKTLANLPWI VEPGQEAKRG INTKYETTIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

510 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.