Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AML1 / RUNX1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118416-100
Catalog Number: LS-G118416
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RUNX1 (NM_001754), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AMLCR1 | Aml1 oncogene | AML1 | AML1-EVI-1 | CBF-alpha-2 | CBFA2 | Acute myeloid leukemia 1 | EVI-1 | PEBP2A2 | PEA2-alpha B | AML1-EVI-1 fusion protein | Oncogene AML-1 | PEBP2-alpha B | PEBP2aB | RUNX1
- Origin Species
- Human
- NCBI GenBank
- NM_001754
- SwissProt
- Q01196
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASDSIFESF PSYPQCFMRE CILGMNPSRD VHDASTSRRF TPPSTALSPG KMSEALPLGA61PDAGAALAGK LRSGDRSMVE VLADHPGELV RTDSPNFLCS VLPTHWRCNK TLPIAFKVVA121LGDVPDGTLV TVMAGNDENY SAELRNATAA MKNQVARFND LRFVGRSGRG KSFTLTITVF181TNPPQVATYH RAIKITVDGP REPRRHRQKL DDQTKPGSLS FSERLSELEQ LRRTAMRVSP241HHPAPTPNPR ASLNHSTAFN PQPQSQMQDT RQIQPSPPWS YDQSYQYLGS IASPSVHPAT301PISPGRASGM TTLSAELSSR LSTAPDLTAF SDPRQFPALP SISDPRMHYP GAFTYSPTPV361TSGIGIGMSA MGSATRYHTY LPPPYPGSSQ AQGGPFQASS PSYHLYYGAS AGSYQFSMVG421GERSPPRILP PCTNASTGSA LLNPSLPNQS DVVEAEGSHS NSPTNMAPSA RLEEAVWRPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
480 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.