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ALDH7A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ALDH7A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129033-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALDH7A1 (NM_001182), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
26g turgor protein homolog | ALDH7A1 | EPD | PDE | Alpha-AASA dehydrogenase | Antiquitin-1 | ATQ1 | Betaine aldehyde dehydrogenase | p6c dehydrogenase
Origin Species
Human
NCBI GenBank
NM_001182
SwissProt
P49419

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSTLLINQPQ YAWLKELGLR EENEGVYNGS WGGRGEVITT YCPANNEPIA RVRQASVADY
61EETVKKAREA WKIWADIPAP KRGEIVRQIG DALREKIQVL GSLVSLEMGK ILVEGVGEVQ
121EYVDICDYAV GLSRMIGGPI LPSERSGHAL IEQWNPVGLV GIITAFNFPV AVYGWNNAIA
181MICGNVCLWK GAPTTSLISV AVTKIIAKVL EDNKLPGAIC SLTCGGADIG TAMAKDERVN
241LLSFTGSTQV GKQVGLMVQE RFGRSLLELG GNNAIIAFED ADLSLVVPSA LFAAVGTAGQ
301RCTTARRLFI HESIHDEVVN RLKKAYAQIR VGNPWDPNVL YGPLHTKQAV SMFLGAVEEA
361KKEGGTVVYG GKVMDRPGNY VEPTIVTGLG HDASIAHTET FAPILYVFKF KNEEEVFAWN
421NEVKQGLSSS IFTKDLGRIF RWLGPKGSDC GIVNVNIPTS GAEIGGAFGG EKHTGGGRES
481GSDAWKQYMR RSTCTINYSK DLPLAQGIKF Q
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

511 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.