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AKAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AKAP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112654-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AKAP7 (NM_016377), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A-kinase anchor protein 9 kDa | A-kinase anchor protein 18 kDa | AKAP-7 isoforms alpha and beta | AKAP-7 isoform gamma | AKAP 18 | AKAP15 | AKAP18 | AKAP7 | PRKA7 isoform gamma | PRKA7 isoforms alpha/beta
Origin Species
Human
NCBI GenBank
NM_016377
SwissProt
Q9P0M2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
36.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MSEEFEANTM DSLVDMPFAT VDIQDDCGIT DEPQINLKRS QENEWVKSDQ VKKRKKKRKD
61YQPNYFLSIP ITNKEIIKGI KILQNAIIQQ DERLAKAMVS DGSFHITLLV MQLLNEDEVN
121IGIDALLELK PFIEELLQGK HLTLPFQGIG TFGNQVGFVK LAEGDHVNSL LEIAETANRT
181FQEKGILVGE SRSFKPHLTF MKLSKSPWLR KNGVKKIDPD LYEKFISHRF GEEILYRIDL
241CSMLKKKQSN GYYHCESSIV IGEKNGGEPD DAELVRLSKR LVENAVLKAV QQYLEETQNK
301NKPGEGSSVK TEAADQNGND NENNRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

326 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.