Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRG2 / GPR64 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G130343-100
Catalog Number: LS-G130343
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR64(ADGRG2) (NM_001184833), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Epididymal protein 6 | G protein-coupled receptor 64 | EDDM6 | G-protein coupled receptor 64 | GPR64 | HE6 | TM7LN2
- Origin Species
- Human
- NCBI GenBank
- NM_005756
- SwissProt
- Q8IZP9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 110.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVFSVRQCGH VGRTEEVLLT FKIFLVIICL HVVLVTSLEE DTDNSSLSPP PEVETTSLND61VTLSLLPSNE TEKTKITIVK TFNASGVKPQ RNICNLSSIC NDSAFFRGEI MFQYDKESTV121PQNQHITNGT LTGVLSLSEL KRSELNKTLQ TLSETYFIMC ATAEAQSTLN CTFTIKLNNT181MNACAVIAAL ERVKIRPMEH CCCSVRIPCP SSPEELEKLQ CDLQDPIVCL ADHPRGPPFS241SSQSIPVVPR ATVLSQVPKA TSFAEPPDYS PVTHNVPSPI GEIQPLSPQP SAPIASSPAI301DMPPQSETIS SPMPQTHVSG TPPPVKASFS SPTVSAPANV NTTSAPPVQT DIVNTSSISD361LENQVLQMEK ALSLGSLEPN LAGEMINQVS RLLHSPPDML APLAQRLLKV VDDIGLQLNF421SNTTISLTSP SLALAVIRVN ASSFNTTTFV AQDPANLQVS LETQAPENSI GTITLPSSLM481NNLPAHDMEL ASRVQFNFFE TPALFQDPSL ENLSLISYVI SSSVANLTVR NLTRNVTVTL541KHINPSQDEL TVRCVFWDLG RNGGRGGWSD NGCSVKDRRL NETICTCSHL TSFGVLLDLS601RTSVLPAQMM ALTFITYIGC GLSSIFLSVT LVTYIAFEKI RRDYPSKILI QLCAALLLLN661LVFLLDSWIA LYKMQGLCIS VAVFLHYFLL VSFTWMGLEA FHMYLALVKV FNTYIRKYIL721KFCIVGWGVP AVVVTIILTI SPDNYGLGSY GKFPNGSPDD FCWINNNAVF YITVVGYFCV781IFLLNVSMFI VVLVQLCRIK KKKQLGAQRK TSIQDLRSIA GLTFLLGITW GFAFFAWGPV841NVTFMYLFAI FNTLQGFFIF IFYCVAKENV RKQWRRYLCC GKLRLAENSD WSKTATNGLK901KQTVNQGVSS SSNSLQSSSN STNSTTLLVN NDCSVHASGN GNASTERNGV SFSVQNGDVC961LHDFTGKQHM FNEKEDSCNG KGRMALRRTS KRGSLHFIEQ MTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1001 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.