Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRG2 / GPR64 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120132-100
Catalog Number: LS-G120132
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR64(ADGRG2) (NM_001079859), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Epididymal protein 6 | G protein-coupled receptor 64 | EDDM6 | G-protein coupled receptor 64 | GPR64 | HE6 | TM7LN2
- Origin Species
- Human
- NCBI GenBank
- NM_005756
- SwissProt
- Q8IZP9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 109.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVFSVRQCGH VGRTEEVLLT FKIFLVIICL HVVLVTSLEE DTDNSSLSPP PAKLSVVSFA61PSSNGTPEVE TTSLNDVTLS LLPSNETGVK PQRNICNLSS ICNDSAFFRG EIMFQYDKES121TVPQNQHITN GTLTGVLSLS ELKRSELNKT LQTLSETYFI MCATAEAQST LNCTFTIKLN181NTMNACAVIA ALERVKIRPM EHCCCSVRIP CPSSPEELEK LQCDLQDPIV CLADHPRGPP241FSSSQSIPVV PRATVLSQVP KATSFAEPPD YSPVTHNVPS PIGEIQPLSP QPSAPIASSP301AIDMPPQSET ISSPMPQTHV SGTPPPVKAS FSSPTVSAPA NVNTTSAPPV QTDIVNTSSI361SDLENQVLQM EKALSLGSLE PNLAGEMINQ VSRLLHSPPD MLAPLAQRLL KVVDDIVLQL421NFSNTTISLT SPSLALAVIR VNASSFNTTT FVAQDPANLQ VSLETQAPEN SIGTITLPSS481LMNNLPAHDM ELASRVQFNF FETPALFQDP SLENLSLISY VISSSVANLT VRNLTRNVTV541TLKHINPSQD ELTVRCVFWD LGRNGGRGGW SDNGCSVKDR RLNETICTCS HLTSFGVLLD601LSRTSVLPAQ MMALTFITYI GCGLSSIFLS VTLVTYIAFE KIRRDYPSKI LIQLCAALLL661LNLVFLLDSW IALYKMQGLC ISVAVFLHYF LLVSFTWMGL EAFHMYLALV KVFNTYIRKY721ILKFCIVGWG VPAVVVTIIL TISPDNYGLG SYGKFPNGSP DDFCWINNNA VFYITVVGYF781CVIFLLNVSM FIVVLVQLCR IKKKKQLGAQ RKTSIQDLRS IAGLTFLLGI TWGFAFFAWG841PVNVTFMYLF AIFNTLQGFF IFIFYCVAKE NVRKQWRRYL CCGKLRLAEN SDWSKTATNG901LKKQTVNQGV SSSSNSLQSS SNSTNSTTLL VNNDCSVHAS GNGNASTERN GVSFSVQNGD961VCLHDFTGKQ HMFNEKEDSC NGKGRMALRR TSKRGSLHFI EQMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1003 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.